Yearly Archives: 2024

　　The reason why people live tired, is not fit the shelf, tear can not open face, untied plot (the reason is very simple)At the same time, pu-erh tea It is no longer a relatively unfamiliar industry, and it enters the public’s sight more and more frequently, gaining more attention and recognition.

　　Your life needs to understand the stand in their own position, not everyone is in line with your appetite; standing in other positions, you can also to all the appetite? (Tolerance of others)

　　People who can simplify complex problems are talented people, and it is silly to complicate simple problems. (People are often willing to be a fool)

　　A woman most admired men talented, most hated men are too stingy, most afraid of men do not live up to expectations. (Men afraid of the old woman angry)

　　Itching sometimes worse than pain, hate is often easier than love (paradox of life)

　　The opportunity for everyone is equal, you can not shoot the chance, but the opportunity comes when you can not do without bullets (doing business is to do this)

　　Love is like a smallpox, and each of us has to go through it once, and, as with smallpox, we will only get once in a lifetime. You never have to worry about getting a second time.

　　Ancient love stories and more contemporary love accident (different times).

　　A person who does not make mistakes usually can not accomplish anything, and there are no shortcomings, often with little merit (Lincoln’s experience).

　　Businessmen are “people who can talk,” businessmen are “people who can give birth to ideas.” (Alternative explanation)

　　There are seven virtues: First, do not get the ax, the second is not to interrupt other words, the third is not anxious for success, four questions should be targeted, five answers to meet the reasonable, six is to have a beginning and end, seven is to Based on reality. (Ancient Jewish Proverbs).

　　Unit to work overtime every day; home never stick; mobile phone home to close; SMS reading on the delete; go to bed snoring shaking; underwear often wear back. (With an affair of the six symptoms).

　　Do what you think can not do (how much heart, how far the road).

　　The heart is a two-bedroom house, one is suffering, one is happy. People can not laugh too loud, or laughter will wake up next to the pain. (To avoid very happy).

　　If you marry a wise and virtuous wife, you will be happy; if you marry a frivolous bitch, you will become a philosopher. (Marriage useful).

　　Can not tell, can not cry, is the most bitter (life reflected).

　　Where to fall, just lie down (some pessimists).

　　The real question is not whether the spouse is the best person, but whether you are trying to be the best person for your spouse (the Happy Marriage Bible).

　　The only advantage of telling the truth is that you do not have to remember what you said (Quotations User).

　　You can offend busy people, because they do not have time to care about you; you must not offend idlers, because they have time to deal with you. (Do not offend idlers).

　　If you can not change your face, then you change your face (not beautiful, it can be very cute).

　　Than bathing, washing the sauna is more important is the brainwashing, bath does not wash will be distributed odor, brain wash will be eliminated by the times (we often forget this).

　　Do not believe your memories, the person inside, not necessarily the same miss you (with memories of the road, rather than holding the memories of life).

　　There should be a better way to start a new day than waking up every morning (office worker philosopher).

　　Toad want to eat swan, good kind, small things with ambition (to educate the next generation).

　　Rich does not have much, but less luxury. (New view of wealth).

　　Most protein is like a jumble of atoms packed together. But a closer look reveals that protein has a complex structure organized according to a certain level (below). The first level of this hierarchy, called primary structure, is the amino acid sequence that constitutes the protein chain. Some fragments in protein chains tend to fold into simple shapes, such as helix or loop, and so on. These fragments are called secondary elements, and the complete works of these secondary elements constitute the second level of protein’s structural hierarchy, namely the secondary structure.in other words recombinant proteins It is possible to develop in a good direction, and there are still many places worth looking forward to in the future. https://www.alphalifetech.com/

　　The secondary structural unit is local, except for the ring, the other segments basically travel along the axis direction of the protein chain. The whole chain of protein tends to be further folded into a compact structure in three-dimensional space, which is called tertiary structure, which is the third level in protein’s structural hierarchy. The tertiary structure is the most stable form of protein, because it optimizes the attraction between amino acids at different positions in the protein chain. Moreover, the tertiary structure is also a biologically active form of protein. If this form is destroyed, protein will lose some or all of its functions. Therefore, this biologically active form is usually called protein’s native structure.

　　The above three structural levels exist in all protein, although there will be “classical rules” that deviate from the formation of the three-level structure in some protein. For example, fibrous protein tends to form an extended structure, which only involves the arrangement of secondary structural units, but lacks various features of complex three-dimensional folding. Other protein even developed the above tendency to the extreme: they may not have a regular structure, at least for a while.

　　The amino acid sequence analysis of protein polypeptide chain, that is, the determination of protein primary structure, mainly includes the following steps:It is strictly required by such a standard, inhibitors Only with today’s development scale, can we have the proud momentum to crush our competitors. https://www.all-chemistry.com/

　　1. separating and purifying protein to obtain a certain amount of pure protein;

　　2. Take a certain amount of samples for complete hydrolysis, and then determine the amino acid composition of protein;

　　3. Analyze the N- terminal and C- terminal amino acids of protein;

　　4. Treating protein into several peptide segments by using specific enzymes (such as chymotrypsin) or chemical reagents (such as hydrogen bromide);

　　5. separating and purifying a single peptide segment;

　　The amino acid sequence of each peptide was determined. Edman degradation method is generally used, and phenyl isothiocyanate is used to react, and amino acids are degraded and determined one by one.

　　7. Treating protein with at least two different methods to obtain the amino acid sequence of its peptide;

　　8. Compare the amino acid sequences of two different peptide groups to obtain the complete amino acid sequence of protein molecule.

　　The first is “poverty”Even if there are obstacles to moving forward, pu-erh tea We should also persevere, forge ahead bravely, cut waves in the sea of the market, hang on to Yun Fan and strive for the first place.

　　Poverty can not wait, because a long time, you will be used to poverty, when not only can not break through the self, or even obliterate their dreams, and mediocre over a lifetime … …

　　The second is “dream”

　　Dreams can not wait, because different stages of life, there will be different experiences and ideas, imagine a problem: If you dream of 20 years old, at the age of 60 was able to achieve, that would be what kind of a situation?

　　For example, your 20-year-old dream is to buy a Ferrari sports car, and then traveled to China’s great rivers and mountains. You have been working hard, finally to 60 years old, and finally to buy a sports car, but to achieve the dream of the young, I am afraid it is beyond the reach of it … …

　　The third is “family”

　　Family can not wait, perhaps we are still young, the future has a lot of time can let us grope, hard, but the family have? They still have time for us to succeed? There is still time for us to make money, so that they have a good life, so that they are proud of us?

　　Trees for static and wind, the child wants to support and pro-not wait. This is a lot of people’s pain, but also a lot of people a lifetime of regret!

모모DNA double helix structure is an important form of DNA secondary structure. It is a structural model put forward by Watson and Crick in 1953.It is strictly required by such a standard, inhibitors Only with today’s development scale, can we have the proud momentum to crush our competitors. https://www.all-chemistry.com/

모모

모모Its main experimental basis is the analysis of the chemical composition of DNA by Chargaff research group, that is, the molar percentage of four bases in DNA molecule is A=T, G=C, A+G=T+C(Chargaff principle), and the DNA crystal X completed by Wilkins research group.

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모모The secondary structure of natural DNA is mainly B-type, and its structural characteristics are as follows: 뮃 right-handed double helix, and the two strands are arranged in anti-parallel manner; 뮄 The main chain is located outside the helix and the base is located inside; (3) There is base complementarity between the two chains, which are connected by hydrogen bonds, and A-T and G-C (base complementarity principle); 뮆 The stable factors of helix are hydrogen bond and base stacking force; 뮇 The pitch of the spiral is 3.4nm and the diameter is 2nm.

　　Protein is a substance with a certain spatial structure, which is formed by the zigzag folding of polypeptide chains composed of amino acids in the form of dehydration and condensation. However, natural protein molecules are not random loose polypeptide chains. Each natural protein has its own unique spatial structure or three-dimensional structure, which is usually called the conformation of protein, that is, the structure of protein. The sequence of amino acids in protein molecule and the three-dimensional structure formed from it constitute the diversity of protein structure.Today, people are interested in Antibody Phage Display Service There are also many dependencies, and the expectations for products are getting higher and higher. https://www.alphalifetech.com/

　　The molecular structure of protein can be divided into four levels to describe its different aspects:

　　Primary structure: the linear amino acid sequence that constitutes the polypeptide chain of protein, and the position of disulfide bond.

　　Secondary structure: In the local area of protein molecule, the polypeptide chain is coiled and folded along a certain direction.

　　Tertiary structure: The three-dimensional structure of a protein molecule formed by the arrangement of multiple secondary structural elements in three-dimensional space.

　　Quaternary structure: used to describe protein complex molecules with biological functions formed by the interaction between different polypeptide chains (subunits).

　　In addition to these structural levels, protein can be transformed in several similar structures to exercise its biological functions. For functional structural changes, these tertiary or quaternary structures are usually described by chemical conformations, and the corresponding structural transformations are called conformational changes.

　　Summer dusk, I sit with an old Chinese medicine in the breeze slowly chatting the river. Suddenly, the old man stared at my face for a moment and did not say anything. I was his heart to see some hair, asked: “The elderly, you are so concerned about my face, is not something to see ah?Even so, pu-erh tea We must also adhere to the quality of the industry and create unique products for the company.

　　Old man smiled and said: “I am from primary school medicine, the teacher told me again and again, ‘people peace of mind level, see the eyes; life span, look at the face’ medical life, develop habits, and Oh, and people Together, they always want to look at his face more glances.

　　I said: “The elderly, all know that the eyes are the soul of the window, the first sentence I can understand, you can say ‘life span, look at the face’, I am a little puzzled.Human life, how to write in the face What about it? “

　　If a person is always sad intestine knot, mood melancholy, over-thinking, suspicious of the old man said: \ look at the face, in fact, I look at his face two, one eyebrow, Indecisive, can not see the hope of life and the good life, then, between the eyebrow must form a deep ‘eight’ pattern.’8 ‘pattern, is caused by squeezing distortion, Yin is too strong, the mood Difficult to cool, it will stagnate in the organs; Jiuyin poisonous, viscera unbearable corrosion, must be made for the disease; sick in the body, is bound to further deteriorate the mood.People once caught in the heart and the destruction of each other malignant disease Circle among the health does not exist, where there will be longevity of the reason? Eyebrow between the ‘eight’ pattern, in fact, cut the knife of life, so my teacher had more than once said to me, ‘brow wrinkled, You must be careful yo. “

　　The elderly, then let my heart anxious, usually not pay attention to the eyebrow between the ‘eight’ pattern, even with such a large knowledge. I could not help but curiosity driven, and then asked: “That mouth can see what?

　　A person if the hearty and fun, often laughing more than, the teeth often in the sun, then, in order to make the mouth a little bit, The ‘brackets’ is radian, as if the relative out of the two and a half months, set in the cheek on top of the vivid expression of light from the formation of the reasons we can see, brackets who are really comfortable Because the ‘brackets’ on both sides of the mouth, but also a symbol of the balance of yin and yang, therefore, it is encompassed by the health of the body, the spirit of the sparse, the vitality of life, leisurely and carefree, The music of the state of mind and the endless hope of life in such a life and mental state of the people, you say he can not live longer?

　　Really listen to Jun words, read the book a decade, my heart is not good fun ah. It turned out that our face, really reveal the mentality of our strengths and weaknesses, the vicissitudes of life and life status and other important information ah. Therefore, we usually still often look in the mirror to see if his face is increasing or deepening: the eyebrow between the “eight” pattern, or the mouth “brackets” it!

　　Separation and purification of protein;The data shows that, Chemical Custom synthesis Its development potential should not be underestimated, and it is also the inevitability of its existence. https://www.all-chemistry.com/

　　1. Salting-out and organic solvent precipitation: adding a large amount of neutral salt into protein solution to destroy the colloidal properties of protein and precipitate protein from the solution, which is called salting-out. Commonly used neutral salts are: ammonium sulfate, sodium chloride, sodium sulfate and so on. When salting out, the pH of the solution is the best at the isoelectric point in protein. Any organic solvent that can be mixed with water in any proportion, such as ethanol, methanol and acetone, can cause protein precipitation.

　　2. Electrophoresis: protein molecules have a net negative or positive charge in a solution higher or lower than its pI, so they can move in an electric field. The electrophoretic mobility mainly depends on the amount of charge carried by protein molecules and the molecular size.

　　3. Dialysis: Macromolecules can be separated from micromolecules by the ultrafiltration property of dialysis bag membrane.

　　4. Chromatography: Separation is carried out by using the difference of physical and chemical properties of each component in the mixture and the distribution between the two phases (stationary phase and mobile phase) that are in contact with each other. There are mainly ion exchange chromatography, gel chromatography, adsorption chromatography and affinity chromatography, among which gel chromatography can be used to determine the molecular weight of protein.

　　5. Ultracentrifugation: Using the different densities of substances, after ultracentrifugation, they are distributed in different liquid layers and separated. Ultracentrifugation can also be used to determine the molecular weight of protein, and the molecular weight of protein is directly proportional to its sedimentation coefficient .

　　Protein is a substance with a certain spatial structure, which is formed by the zigzag folding of polypeptide chains composed of amino acids in the form of dehydration and condensation. However, natural protein molecules are not random loose polypeptide chains. Each natural protein has its own unique spatial structure or three-dimensional structure, which is usually called the conformation of protein, that is, the structure of protein. The sequence of amino acids in protein molecule and the three-dimensional structure formed from it constitute the diversity of protein structure.As it happens, many people are killed recombinant proteins And bring more benefits, make it flourish, and promote the industry greatly. https://www.alphalifetech.com/

　　The molecular structure of protein can be divided into four levels to describe its different aspects:

　　Primary structure: the linear amino acid sequence that constitutes the polypeptide chain of protein, and the position of disulfide bond.

　　Secondary structure: In the local area of protein molecule, the polypeptide chain is coiled and folded along a certain direction.

　　Tertiary structure: The three-dimensional structure of a protein molecule formed by the arrangement of multiple secondary structural elements in three-dimensional space.

　　Quaternary structure: used to describe protein complex molecules with biological functions formed by the interaction between different polypeptide chains (subunits).

　　In addition to these structural levels, protein can be transformed in several similar structures to exercise its biological functions. For functional structural changes, these tertiary or quaternary structures are usually described by chemical conformations, and the corresponding structural transformations are called conformational changes.

　　Summer dusk, I sit with an old Chinese medicine in the breeze slowly chatting the river. Suddenly, the old man stared at my face for a moment and did not say anything. I was his heart to see some hair, asked: “The elderly, you are so concerned about my face, is not something to see ah?Today, people are interested in pu-erh tea There are also many dependencies, and the expectations for products are getting higher and higher.

　　Old man smiled and said: “I am from primary school medicine, the teacher told me again and again, ‘people peace of mind level, see the eyes; life span, look at the face’ medical life, develop habits, and Oh, and people Together, they always want to look at his face more glances.

　　I said: “The elderly, all know that the eyes are the soul of the window, the first sentence I can understand, you can say ‘life span, look at the face’, I am a little puzzled.Human life, how to write in the face What about it? “

　　If a person is always sad intestine knot, mood melancholy, over-thinking, suspicious of the old man said: \ look at the face, in fact, I look at his face two, one eyebrow, Indecisive, can not see the hope of life and the good life, then, between the eyebrow must form a deep ‘eight’ pattern.’8 ‘pattern, is caused by squeezing distortion, Yin is too strong, the mood Difficult to cool, it will stagnate in the organs; Jiuyin poisonous, viscera unbearable corrosion, must be made for the disease; sick in the body, is bound to further deteriorate the mood.People once caught in the heart and the destruction of each other malignant disease Circle among the health does not exist, where there will be longevity of the reason? Eyebrow between the ‘eight’ pattern, in fact, cut the knife of life, so my teacher had more than once said to me, ‘brow wrinkled, You must be careful yo. “

　　The elderly, then let my heart anxious, usually not pay attention to the eyebrow between the ‘eight’ pattern, even with such a large knowledge. I could not help but curiosity driven, and then asked: “That mouth can see what?

　　A person if the hearty and fun, often laughing more than, the teeth often in the sun, then, in order to make the mouth a little bit, The ‘brackets’ is radian, as if the relative out of the two and a half months, set in the cheek on top of the vivid expression of light from the formation of the reasons we can see, brackets who are really comfortable Because the ‘brackets’ on both sides of the mouth, but also a symbol of the balance of yin and yang, therefore, it is encompassed by the health of the body, the spirit of the sparse, the vitality of life, leisurely and carefree, The music of the state of mind and the endless hope of life in such a life and mental state of the people, you say he can not live longer?

　　Really listen to Jun words, read the book a decade, my heart is not good fun ah. It turned out that our face, really reveal the mentality of our strengths and weaknesses, the vicissitudes of life and life status and other important information ah. Therefore, we usually still often look in the mirror to see if his face is increasing or deepening: the eyebrow between the “eight” pattern, or the mouth “brackets” it!